ACUTE AND SUBCHRONIC TOXICITY OF SEVERAL PHENOLIC UNCOUPLERS OF OXIDATIVE PHOSPHORYLATION IN FATHEAD MINNOWS (PIMEPHALES PROMELAS)
Date
1983Author
Call, D.J.
Poirier, S.H.
Harting, S.L.
Lindberg, C.A.
Northcott, C.E.
Brooke, L.T.
Metadata
Show full item recordAbstract
Acute and early life stage_ (ELS) toxicity tests were conducted with fathead minnows
(Pimephales promela$) and seven chemicals: 2,4-dinitrophenol (2,4-DNP), 4,6-dinitro-o-
cresol (DNOC), 2-sec -butyl--4,6-dinitrophenol (dinoseb) 2,2' methylenebis (3,4,6-trichlorophenol) (hexac lorophene), 2 2'-
methylenebis(4-chlorophenol) (dichlorophen), 3,5-dibromo-4-
hydroxybenzonitrile (bromoxynil) and salicylanilide. Data on pentachlorophenol (PCP) were
available in the literature. Two of the eight chemicals, bromoxynil and salicylanilide, were
notably less toxic than the others in a plot of acute toxicity and lipophilic property
(g-octanol/water partition coefficient). Five of the chemicals appeared to share a common mode of
toxic action, the uncoupling of oxidative phosphorylation: 2,4-DNP, DNOC, dinoseb, PCP, and
hexachlorophene. Although dichlorophen toxicity in both acute and ELS tests appeared to be
similarly dependent upon lipophilicity as the above five chemicals, its high dissociation constant
indicated a somewhat different mechanism. A predictive model was presented for estimating
sub-chronic toxicity based upon partition coefficient. The acute/chronic ratio for the five uncouplers based upon the ELS test endpoint of total biomass per chamber was 4.5 ± 1.0. Using the most sensitive endpoint for the four
uncouplers for which discrete MATC estimates were obtained, the ratio was 16.2 + 7.8.
Subject
acute
subchronic toxicity
phenolic uncouplers
oxidative phosphorylation
Fathead Minnow
Pimephales Promelas
fish
Phenolic uncouplers
acute/chronic ratio