Transient kinetics studies of azo dye oxidation catalyzed by horseradish peroxidase
File(s)
Date
2017-04Author
Jernberg, Beau
Publisher
University of Wisconsin--Stout. Office of Research and Sponsored Programs
Advisor(s)
Miller-Rodeberg, Marcia
Metadata
Show full item recordAbstract
The release of azo dyes into the environment is a continuing hazard
resulting from industrial wastewater being released into the ecosystem. The
industrial wastewater contains azo dye runoff that is used to color textiles,
food, cosmetics, and other industrial products1-3. Oxidative decolorization
is a potential method to remediate azo dye contamination1-3. Horseradish
peroxidase (HRP) is an enzyme with a wide substrate range for organic
compounds and strong oxidative power, making it a potential catalyst for azo
dye decolorization. The oxidative strength of HRP is due to the Fe(III)-heme
prosthetic group that reacts directly with hydrogen peroxide (H2O2) to form
the high valent Fe-oxo species known as compound I (cpdI) and a subsequent
intermediate, compound II (cpdII)4-8. These enzyme-bound high valent Feoxo
species extract single electrons from a range of organic compounds via
a collisional reaction, generating free radical species in solution. The radical
species undergo radical quenching via different chemical routes, resulting
in a wide range of products from a single organic substrate4-8. Previous
research has shown that HRP catalyzes the oxidation of several azo dyes.
The research described here focuses on the oxidation of the azo dye Orange
IV (OIV). Initial research showed that OIV is oxidized to form a product,
denoted as P-1, via a multistep process; however, further work showed that
the reaction continued at a slower rate. The research described here utilized
transient kinetic techniques to examine the slower reaction to elucidate
a more complete mechanism for the oxidation of OIV and aid in future
environmental remediation techniques.
Subject
horseradish peroxidase
azo dye
Permanent Link
http://digital.library.wisc.edu/1793/77587Description
Research article with graphs, tables, and illustrations.
Citation
Jernberg, B. (2017). Transient kinetics studies of azo dye oxidation catalyzed by horseradish peroxidase. University of Wisconsin-Stout Journal of Student Research, 16, 128-142.